Sanger Sequencing

Sanger sequencing, also known as first-generation sequencing, was developed by Frederick Sanger in the 1970s. This method relies on a chain-termination technique using dideoxynucleotides (ddNTP) to accurately determine DNA sequences. Although it has lower throughput compared to modern next-generation sequencing technologies, Sanger sequencing remains the gold standard for accuracy, particularly in small-scale projects and for validating results obtained from high-throughput methods.