This tool uses the following accepted average values to estimate the molecular weight (Mw) of nucleic acids:
- dsDNA: 650 g/mol per base pair
- ssDNA: 325 g/mol per base
- ssRNA: 340 g/mol per base
For oligonucleotides (Oligo), it is recommended to enter the precise molecular weight (in kDa) provided by the supplier.
Core Formulas:
1. Moles (mol) = Mass (g) / Molecular Weight (g/mol)
2. Concentration (M) = Moles (mol) / Volume (L)